Sunflower enzymatic reaction optimization: MAG and DAG formation / Otimização da reação enzimática do girassol: formação de MAG e DAG

Edinara Adelaide Boss, Mariana Leme Calais, Maria Regina Wolf Maciel, Paula Sbaite, Rubens Maciel Filho

Abstract


Monoacylglycerols (MAG) and diacylglycerols (DAG) are emulsifiers widely used in food and cosmetic industries. Typical industrial processes for MAG and DAG production consist of the catalytic glycerolysis of triacylglycerols at high temperatures (>200°C). However, the inorganic catalyst and the high temperature contribute to the formation of a product often dark and with unpleasant smell. The goal of this work is the use of enzymatic glycerolysis to improve product aspect and quality, increase selectivity and decrease energy consumption. This reaction produces a mixture of approximately 20% of MAG and 50% of DAG. In order to evaluate the effects of reaction parameters in the MAG and DAG content after the enzymatic glycerolysis reactions of refined sunflower oil a 23 experimental design was employed. A surface response analysis was used to optimize this process.


Keywords


glycerolysis; sunflower oil; enzyme catalysis; experimental design

Full Text:

PDF

References


Kristensen, J.B., Xu, X., Mu, H., 2006. Diacylglycerol synthesis by enzymatic

glycerolysis: Screening of commercially available lipases. Journal of the Americam Oil

Chemists´ Society, v. 82, n. 5, p. 329-334.

Katsuragi, T., Yasukawa, T., Matsuo, N., Flickinger, B., Tokimitsu, I., Matlock,

M.Diacylglycerol Oil, 2 ed. EUA: AOCS Press, 2004. 272 p.

Schoenfelder, W. Determination of monoglycerides, diglycerides, triacylglycerols and

glycerol in fats by means of gel permeation chromatography [C-VI 5b(02)]. Eur. J.

Lipid Sci. Technol, n.105, p. 45-48, 2003

Fregolente, P. B. L.; Pinto, G. M. F.; Wolf Maciel, M. R.; Maciel Filho, R.

Monoglyceride and diglyceride production through lipase-catalyzed glycerolysis and

molecular distillation. Applied Biochemistry and Biotechnoloy, v. 160, p. 1879-1887,

Boss, E. A., Toledo, E. C. V., Maciel Filho, R., 2004. Freeze-drying Process: real

Time Model and Optimization. Chemical Engineering and Processing. v. 43, p. 1475-1485.

Kalil, S.J., Maugeri F. and Rodrigues M.I., 2000, Response surface analysis and

simulation as a tool for bioprocess design and optimization. Process Biochemistry. v.

, 539-550.




DOI: https://doi.org/10.34117/bjdv6n5-299

Refbacks

  • There are currently no refbacks.