Efeitos da carbamazepina sobre o epidídimo e possíveis impactos sobre a maturação espermática / Epididymal effects of carbamazepine and potential impacts on sperm maturation

Marina Nunes, Leonardo Wensing Fischer, Rafaela Doretto do Valle Freitas, Sandra Maria Miraglia, Samara Urban de Oliva

Abstract


A carbamazepina (CBZ) é um fármaco amplamente utilizado no controle de convulsões, no tratamento da neuralgia do trigêmeo e em desordens afetivas. Alterações das características seminais, dos níveis de hormônios sexuais, da espermatogênese e da fertilidade masculina têm sido referidas durante e após terapia prolongada com CBZ. Embora não existam estudos evidenciando a ação deste medicamento sobre o epidídimo e o processo de maturação espermática, possíveis efeitos diretos da CBZ sobre os espermatozóides epididimários devem ser considerados, pois este fármaco é altamente lipossolúvel. O objetivo deste trabalho foi investigar uma possível ação direta da CBZ sobre parâmetros qualitativos do espermatozóide epididimário, utilizando o rato como modelo experimental, na fase adulta jovem. Foram utilizados ratos machos, da linhagem Wistar, distribuídos em 2 grupos: tratado com CBZ (200mg/kg, via gavagem), por quatro dias consecutivos, a partir dos 91 dias, e controle (n=5) - os animais receberam o veículo (azeite de oliva), seguindo o mesmo protocolo experimental utilizado no grupo tratado. Os testículos e epidídimos esquerdos e a próstata ventral foram removidos e pesados. Espermatozóides foram coletados da porção proximal da cauda epididimária direita e avaliados quanto a motilidade, a vitalidade e a morfologia. O tratamento com CBZ causou redução significante na proporção de espermatozóides com motilidade progressiva associada ao aumento de espermatozóides apresentando motilidade não progressiva. Além disso, houve aumento significativo na porcentagem de espermatozóides mortos e de espermatozóides com alterações morfológicas. Os resultados sugerem que a CBZ pode atuar diretamente sobre o espermatozóide epididimário de ratos adultos jovens, reduzindo a fertilidade masculina.


Keywords


Epidídimo; Carbamazepina; Espermatozóides

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DOI: https://doi.org/10.34119/bjhrv3n3-017

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